Development of a novel drug target-profiling platform in yeast


Project description
A large variety of anti-cancer drugs and pharmacophores used in the clinic (e.g. Jaspines, NPC-inducing agent U18666A, Miglustat) interfere with sphingolipid homeostasis, but their mechanism of action and/or potentially deleterious side effects are not known. Using a yeast three-hybrid assay to screen for small molecule-protein interactions, we will set up new technology to permit proteome-wide screening of membrane protein-drug interactions, thus facilitating identification of the molecular targets of Jaspines and other sphingolipid-deregulating compounds.

Further reading
Kittanakom et al, 2009, Methods Mol Biol, 548:247-71
Möckli et al, 2007, Biotechniques, 42(6): 725-30
Becker et al, 2004, Chem Biol, 11(2): 211-23
Chidley et al, 2011, Nat Chem Biol, 7(6): 375-83

Host institute
Institute of Chemical Sciences and Engineering
École Polytechnique Fédérale de Lausanne
CH-1015 Lausanne, Switzerland
Phone: +41 (0)21 6939356

The host institute is one of the top-ranked European Technical Universities, located at the shore of beautiful Lake Geneva. It possess an excellent infrastructure and a highly international faculty and student population.

•   We are looking for an ambitious and interactive individual with PhD degree in molecular biology or microbiology. Since the focus of the project is technology development strong skills for method establishment is a must. Work experience with cell culture, cell transfection and protein expression is an advantage. Prior experience with yeast is an asset.
•   Proactive participation in SPHINGONET’s research & training program is expected through presentations and critical thinking at internal scientific forums, requiring excellent English presentation and writing skills. Strong communication skills and the ability to work in a team are a must.
•   Candidates must fulfill the eligibility criteria for Marie Curie Initial Training Networks (FP7).
•   Candidates should submit the complete application package.
•   Suitable candidates will be invited for an interview by the supervisor of the project.

•    PostDoc for 2 years.
•    Full-time (42 hours/week).